Veterinary Immunology Forum

Jackson ImmunoResearch Laboratories, Inc

2007-03-18T13:41:00.015-05:00

Services: Secondary antibodies
URL:http://www.jacksonimmuno.com/
Jackson ImmunoResearch Laboratories, Inc.
P.O. Box 9872
West Baltimore Pike
West Grove, PA, USA 19390
Telephone:1-800-FOR-JAXN (367-5296)
Fax:610-869-4024

http://www.jacksonimmuno.com/home/distasia.asp

SouthernBiotech

2007-03-18T13:41:00.014-05:00

Services: Veterinary antobodies

http://www.southernbiotech.com/

P.O. Box 26221

Birmingham,

Alabama 35260

USA

Phone: (205) 945-1774

FAX: (205) 945-8768

info@SouthernBiotech.com

Toll-free within the US and Canada 1-800-722-2255

More:http://www.southernbiotech.com/distributormap.htm

Abd Serotec

2007-03-18T13:41:00.013-05:00

Services: Veterinary antibodies, custom monoclonals, OEM and custom services

USA & Canada:
3200 Atlantic Avenue,

Suite 125Raleigh,

NC 27604,

USAToll free: 1-800-265-7376

Fax: 919-878-3751

sales.us@ab-direct.com

India:

NuLife Consultants & Distributors (Pvt.) Ltd.

L-33, First Floor,

Lajpat Nagar- Part-II,

New Delhi-110024-India

Ph.No.91-11-29816141 /29816151/ 29816161

Fax - 91 -11-29816151

nulife@bol.net.in www.nulife.in

More at:http://www.ab-direct.com/about/distributors-403.html

Veterinary Medical Research & Development, Inc.

2007-03-18T13:41:00.012-05:00

Services: Test kits, Immunoflurescence reagents, antibodies, diagnostics services,
URL:http://www.vmrd.com/
Phone: 1-509-334-5815 • 1-800-222-8673
Fax: 1-509-332-5356
E-mail: vmrd@vmrd.com
Services: Test kits, Immunoflurescence reagents, antibodies, diagnostics services,

Veterinary Immunology: An Introduction, 7th Edition

2007-03-18T13:41:00.011-05:00

Veterinary Immunology, 7th Edition - An Introduction By Ian R. Tizard, PhD, BSc, BVMS

Veterinary Immunology: An Introduction has been the veterinary student's book of choice since 1977. The 7th edition continues this tradition and has the added benefit of full-color photos and important new information. Still an excellent first-level textbook for students of veterinary and comparative medicine, this text also assists veterinarians by informing them about recent developments in immunology as they relate to domestic animals.

Key Features:
• Straightforward text clearly explains the general principles of immunology

• Includes information on the most significant immunological diseases and immunological responses seen in domestic animals

• Includes clinical examples to illustrate all of the principles discussed

• It’s the only complete resource on the subject

• Expanded and updated references to all of the key sources in the literature

Vaccine

2007-03-18T13:41:00.010-05:00

VACCINE is the pre-eminent journal for those interested in vaccines and vaccination. It serves as an interface between academics, those in research and development, and workers in the field. Relevant topics range from basic research through to applications, safety and legislation.

Key aspects include

• human

• veterinary

• disease prevention

• physiological manipulation

• molecular biology

• synthetic peptides

• recombinant antigens

• vectors

• new immunogens

•adjuvants

•animal models

• immunity

•immunology of protection

• fertility

•academic research

•developmental applications

• field trials

• clinical trials

• laboratory productions

• industrial production

• social implications

• epidemiology

• efficacy

• safety

• legislation

• regulation

• cost/benefit

These aspects may be applied to diseases caused by: viruses, bacteria, mycoplasma, protozoa, fungi, helminths, arthropods,prions

Comparative Immunology, Microbiology and Infectious Diseases

2007-03-18T13:41:00.009-05:00

Comparative Immunology, Microbiology and Infectious Diseases is an international journal for medical and veterinary researchers and Practitioners. It has an Impact Factor of 1.562 and is ranked 13th out of 129 Veterinary Science titles in the 2005 ISI Journal Citation Reports!

There is a tendency for physicians and veterinarians interested in Immunology, Immunopathology, Microbiology and Infectious Diseases to publish in specialist journals on human or animal medicine. Consequently they are often unaware of each others problems. Yet is widely recognized that interdisciplinary cooperation between specialists in human and animal medicine is of interest and benefit to each.

Comparative Immunology, Microbiology & Infectious Diseases fills the gap between these two branches of medicine and enables fruitful dialogues to take place.

The journal is open to subjects of common interest related to Immunology and Immunopharmacology, Microbiology and human and animal Infectious Diseases. The subject-matter is not restricted entirely to Comparative medicine, but every article will contribute further knowledge in fields of common interest to specialists in either branch of medicine.

BMC Veterinary Research

2007-03-18T13:41:00.008-05:00

BMC Veterinary Research is an open access journal publishing original peer-reviewed research articles in all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health. BMC Veterinary Research (ISSN 1746-6148) is indexed/tracked/covered by PubMed.

Manuscripts must be submitted to BMC Veterinary Research electronically using the online submission system.

Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression

2007-03-18T13:41:00.007-05:00

Author(s): Pratip K Chattopadhyay
Affiliation(s): Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
DOI: 10.1038/nprot.2006.1

This protocol details a method to identify CD4+ T cells that respond to antigens. The method relies on detection of CD154, a costimulatory cell surface protein that is expressed by CD4+ T cells upon activation, and can be used to purify live CD4+ T cells of diverse function. To detect CD154, fluorescently labeled antibodies are cultured with cell samples, peptides (or whole antigens) and monensin during a 6- to 24-h stimulation period. (Note that the assay is not compatible with brefeldin A.) After stimulation, cells are stained with any other antibodies of interest and then are analyzed by flow cytometry or purified by cell sorting. Unlike other assays, this method allows simultaneous assessment of other cell phenotypes or functions, is compatible with downstream RNA-based assays and preserves cell viability. This protocol can be completed in 9 h.

Full text
PDF (785K)

Intracellular cytokine optimization and standard operating procedure

2007-03-18T13:41:00.006-05:00

Author(s): Laurie Lamoreaux
Affiliation(s): Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health
DOI: 10.1038/nprot.2006.268

We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and discuss how to procedurally optimize key steps in the experimental process before an intracellular cytokine staining assay protocol is finalized. These include but are not limited to the titration of monoclonal antibodies, use of a dead-cell discriminator and 'dump' channel, selection of a cytokine secretion inhibitor, selection of fixation and permeabilization reagents, and inclusion of compensation controls. Building on this basic protocol, we then establish a polychromatic assay designed to detect five separate functions of T lymphocytes (production of three cytokines and one chemokine, and degranulation) while simultaneously identifying multiple surface markers on the responding cells.

Full text
PDF (417K)

Isolation and purification of afferent lymph dendritic cells that drain the skin of cattle

2007-03-18T13:41:00.005-05:00

Dendritic cells (DCs) are central to the induction of immune responses and are a pivotal control point that determines the outcome of infectious challenge. Cannulation of afferent lymphatic vessels allows the isolation of large numbers of lymph DCs. First, lymph nodes that are draining the skin are surgically removed (takes approximately 1 h). Over a period of 6–8 weeks, afferent lymphatic vessels re-anastomose with the efferent duct, forming larger 'pseudoafferent' lymphatic vessels that can be surgically cannulated. Surgical cannulation takes 2 h to perform; daily maintenance of the catheter requires 30 min. Isolation of lymph cells requires 1 h and an additional 60–180 min to enrich or purify the DCs. The lymph can be harvested for up to 1 month, with relatively constant cell numbers and subset distribution throughout this period. This technique, although technically demanding, facilitates studies of DCs and other cells that traffic in the lymph in both the steady state and following antigenic exposure.

Full text
PDF (292K)

A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators

2007-03-18T13:41:00.004-05:00

Innovative therapies for severe lung diseases (such as allergic and chronic asthma, chronic obstructive pulmonary disease or any type of lung cancer) require a detailed understanding of the cellular and immune processes in the lung. This protocol details a method to obtain the immune cells of the bronchi as well as the cytokines and mediators produced by these cells for further investigation. The broncho-alveolar lavage fluid (BALF) is taken by injecting physiological solution through the tracheal tube into the murine airways and carefully regained by winding up the connected syringe. After centrifugation, the resulting BALF supernatant can be stored for detection of cytokines or other mediators by enzyme-linked immunosorbent assay or other methods; the resuspended cell pellet can also be used for flow cytometric analyses, to check cell viability and the level of apoptosis, as well as other applications. In addition, CD4+ T cells isolated from wild-type and genetically modified mice alone or along with other immunologically important cells such as T regulatory cells, which can be used to reconstitute immunodeficient mice, may be retrieved from the airways with this method. This protocol can be completed within 35 min.

Full text
PDF (394K)

Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung

2007-03-18T13:41:00.003-05:00

The regulation of the cellular immune response in lung diseases is not yet fully understood. Isolating different subsets of immune cells directly from the lung is therefore an indispensable method of gaining detailed knowledge on the function of these cells in this organ. This protocol describes a method of isolating and magnetically labeling CD4+ lung T cells, which are then loaded and retained on the column while all other cells run through it (positive selection). The yield of this isolation is approximately 5 × 10^5 to 1.5 × 10^6 CD4+ cells from a murine lung. These cells can be further investigated by several methods such as flow cytometry, western blot analysis, RT-PCR, immunostaining and ELISA. In addition, lung CD4+ T cells alone or along with other immunologically important cells such as CD8+ T cells and T regulatory cells can be adoptively transferred into immune-deficient mice, and can influence important local parameters. This protocol can be completed in approximately 4 h 20 min.

Full text
PDF (409K)

Immunobiology : the immune system in health and disease

2007-03-18T13:41:00.002-05:00

Read here

American Association for Veterinary Immunologists

2007-03-18T13:41:00.001-05:00

The American Association of Veterinary Immunologists (AAVI) is dedicated to the development, promotion, and dissemination of knowledge in veterinary immunology. This includes immunology of livestock and poultry, companion animals, fish and marine mammals. The AAVI was formed in 1979 by a group of veterinary immunologists assembled at the annual Conference of Research Workers in Animal Diseases (CRWAD). The AAVI is open to all veterinary immunologists internationally The AAVI holds an annual business and scientific meeting in conjunction with CRWAD each year as well as an annual symposium in conjunction with the American Association of Immunologists (AAI). The AAVI website outlines many of its activities.

Advantages of AAVI Membership:

1. Sharing of developments within veterinary immunology

Contacts with interested scientists in the US and worldwide through sponsored meetings
Access to vetimm listserv [to send a message just email to vetimm@vasci.umass.edu. To subscribe, or to change your own subscription (i.e. when you get a new email address), go to the following web page: http://list.umass.edu/mailman/listinfo/vetimm]

2. Sponsorship and organization of veterinary immunology programs within numerous meetings, CRWAD, AAI, International Veterinary Immunology Symposium (IVIS)

AAVI/ACVM cosponsored Symposium at CRWAD
AAVI/AAI veterinary immunology committee (VIC) cosponsored symposium at AAI
Lower registration at AAI [$100 savings for registration because AAVI is an AAI Guest Society]
Members of AAVI can become members of the international veterinary immunology committee (IUIS VIC) and of AAI VIC.
Interactions with European Veterinary Immunology Groups (EVIG) and participation in their workshops at European Congress of Immunology
Outreach and interactions with Comparative Immunology societies

3. Development of opportunities for improved veterinary immunology research

The initiative for the National Veterinary Immune Reagent Network was started by AAVI at a USDA ARS veterinary immunology workshop.
This stimulated a national USDA CSREES “Toolkit” grant initiative. An award of $2.15-million was launched February 7, 2006 with Dr. Cynthia Baldwin, University of Massachusetts-Amherst, as the Lead.
The Network is described in the April 2006 ARS Healthy Animals Newsletter www.ars.usda.gov/is/np/ha/han25.htm
Advantages for students, postdoctoral fellows and younger faculty
Presentation awards for students at CRWAD
Travel grants to meetings, e.g., International Veterinary Immunology Symposia (IVIS)
Service on AAVI committees as aid to tenure for new faculty members

4. Mentoring Opportunities

AAVI members will be asked to sign up as potential mentors so they could be teamed up with younger AAVI members.
Younger scientists could interact with faculty from other institutions who could assist with reading manuscripts ready for journal submission, outlines and drafts of grants, etc.
Advise and foster collaboration and reagent sharing between members.

5. Access to journals

electronic subscription for Veterinary Immunology Immunopathology \
service to our organization with meeting announcements and summaries
interest in special issues sponsored by AAVI members
Potential for access to Developmental and Comparative Immunology, another Elsevier journal.

6. Contact with USDA ARS and CSREES leaders concerning community needs

AAVI Immune Toolkit grant and international toolkit efforts
AAVI responses at USDA ARS and CSREES stakeholder workshops

Membership:

Download a membeship application form from here and send it to:

Eileen L. Thacker, DVM, PhD, Diplomate ACVMSecretary-Treasurer, AAVI2118 Vet Med BldgVeterinary Microbiology and Preventive MedicineIowa State University, Ames, IA 50011Phone: (515) 294-5097FAX: (515) 294-8500ethacker@iastate.edu

Veterinary Immunology and Immunopathology

2007-03-18T13:41:00.000-05:00

Veterinary Immunology and Immunopathology is an An International Journal of Comparative Immunology and was started in 1980. The journal publishes immunological studies in animals and birds that are useful to man. The journal has an impact factor of 1.6 and is ranked 12th out of 129 Veterinary journals. The journal cover wide areas of research including basic and applied aspects of Veterinary Immunology. The present
Editors-in-Chief are Drs. C.L. Baldwin, C.J. Howard, and J. Naessens. Free online access to this journal is available to institutions in developing countries via the the AGORA Initiative (set up by the FAO) and the HINARI Initiative (set up by the WHO).

Compensation in flow cytometry

2007-01-04T23:51:00.000-05:00

Mario Roederer, Director, Flow cytometry core facility
at Vaccine Research Center of NIAID, has written a detailed tutorial on Compensation in flow cytometry.

He has effectively discussed about the process of compensation and how it works in flow cytometry. He has also explained the importance of compensation in multicolor flow cytometry. His tutorials are well illustrated with figures and examples.

Tutorials are excellent for anyone who is engaged in multicolor flow cytometry and wants to get rid off the unwanted "spillovers".

For more, visit his tutorial webpage "Compensation: The gruesome details"

8th International Veterinary Immunology Symposium

2007-01-03T23:43:00.000-05:00

Dear Fellow Veterinary Immunologists,

Brazil and the Brazilian Society for Immunology invite you to the 8th International Veterinary Immunology Symposium. It will take place on August 16th-19th 2007, at the Estalagem das Minas Gerais Convention Center. Scientific themes of interest for all areas of veterinary immunology and the beauty of Ouro Preto, a UNESCO World Heritage City, promise to make the 8th IVIS an exciting meeting.

Veterinary Immunologists need not be reminded that our field is the most important basis of knowledge for the development of technologies to promote healthier animals, safer products and international trade without sanitary barriers. The scientific program for the 8th IVIS aims to highlight the contributions that Veterinary Immunology makes towards developing new insights about the Immune System in general. It also aims to bring new concepts that are being developed in Human and Basic Immunology and that may be applied towards understanding of the immune systems from domestic and wild animals.

The program also aims to present the latest research on aspects of immunology that are relevant to animal production systems, such as reproduction, stress and nutrition, which may contribute towards improving these systems and the quality of products they provide. Finally, the program will acquaint participants with the latest developments concerning immune responses, protective or pathological, elicited against prions, microbes and parasites and the interventions that are being developed to control them. Application of modern concepts of Immunology and related fields towards the rational development of vaccines and adjuvants will also be emphasized.

The Local Organizing Committee is striving to derive the maximum information possible from the posters submitted to the IVIS. Posters will be left for viewing during the entire duration of the Symposium as in previous meetings, and the time available for poster exhibition in the presence of presenters has increased. Summaries will be cross-referenced in the program book to facilitate retrieval of information from each area of interest. Key words will therefore be required and, together with summaries, should be online several weeks before IVIS begins. We hope these measures will increase the interactions between presenters and the other participants.

The organizers of the 8th IVIS will continue the tradition of a close alliance with the International Congress of Immunology, which will be held in spectacular Rio de Janeiro, beginning four days after the end of IVIS.

We look forward to seeing you in Ouro Preto,

Isabel de Miranda Santos, 8th IVIS Chair
Beatriz Rossetti Ferreira, 8th IVIS Vice-Chair

Let 2007 be the year of Open Access.

2007-01-02T23:33:00.001-05:00

Let us welcome the 2007 year.

Let us all wish that 2007 would be the year of open access to peer-reviewed scientific literature.

Scientific Content - We, the scientific community, create it. Let us share it openly.

Knowledge - More it is shared - the more it grows and makes humans differ from other animals.

So friends, let us resolve to promote and support Open Access in 2007.

Are you wondering what is Open Access? -

Open-access (OA) literature is digital, online, free of charge, and
free of most copyright and licensing restrictions. What makes it
possible is the internet and the consent of the author or
copyright-holder.

Sharing and contributing in a collaborative manner makes Open Access work. So contribute whatever you can. Start with this blog itself.

Wish you a very Happy New Year.

Veterinary Immunology Forum

2006-12-27T14:30:00.000-05:00

Welcome to our new website on Blogger!

Veterinary Immunology Forum took off as a volunteer project in India in 2004. Since then, we have grown into a fairly large group of like-minded people who are interested in basic and applied aspects of veterinary immunology / comparative immunology. We encourage students and scientists from the immunology and related fields to develop this blog as a resource and information portal for veterinary immunology.

This Blogspot project intends to collect and share resources on Veterinary Immunology from all over the world. Send an email to us if you want to join as a blogger.

Click here to visit our fully functional forum and its objectives.

Courtesy: Special thanks to Ramani for his advice and help for the designing of this blog.